AP Biology Enzyme Synthesis Lab Report

Enzyme Catalysis         Introduction and Overview- In this lab, we will observe the reincarnation of enthalpy peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this conversion response. The catalase enzyme acts as catalysis, helping lower the energy needed to activate the reaction while the enzyme itself is not affected. The reaction could press place without the help of catalase, but it would happen a gage more slowly because more energy is needed for the reaction.

        The nucleus being acted on by the enzyme is known as the substratum, and in this case is hydrogen peroxide. The enzyme changes these subst esteems into products, in this case water and O2 gas. The mean of this lab is to observe and measure the rates of conversion of hydrogen peroxide to water and oxygen by the enzyme catalase over polar periods of time.

        To measure the rate of the reaction, we measure the rate of disappearance of the substrate of over time. Potassium permanganate (KMn04) is used to indicate the presence of each remain H2O2 in the solution after the reaction. The amount of remaining substrate is an indication of how often the conversion reaction took place.

        after allowing an enzyme to act on a substrate solution for assorted periods of time and measuring the amount of remaining substrate, a rate of reaction can be calculated. And that is what the experiment boils down to, calculation out the rate of an enzyme catalyzed reaction.



I predict that the rate for reaction will remain fairly constant over time.

        Materials- · burette         Â· 2% KmnO4                  Â· 1M H2SO4 (Sulfuric acid)         Â· H2O (Water)         Â· Potato         Â· 1.5% H2O2 (Hydrogen Peroxide)         Â· Catalase         Â· 1 ml syringe                  Â· 10 ml syringe         Â· Stopwatch         Â· Six 50 ml beakers         Â· 2 more...

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